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KMID : 1094020070240040603
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Journal of Veterinary Clinics
2007 Volume.24 No. 4 p.603 ~ p.607
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Sample size of pooled sera for detection of chicken infectious bronchitis virus infection
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Pak Son-Il
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Abstract
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The sample sizes required to detect at least one chicken infectious bronchitis virus(IBV) infection at flock-level were determined using pooled samples for 48 submissions with different samples in each. A total of serum samples of 9,980 layers from Kangwon, Chungpook and Chungnam province were collected and tested hemagglutination inhibition(HI) antibody titers against IBV both individually and with pooling size of 10. Of the 48 submissions, 72.9% were required less than 5 pools to detect at least one infected pool at 95% confidence level, and the corresponding rate was 77.1% at 90% confidence level. Overall, the number of pools was decreased as the percent of positive pools increased. At two different cut-of HI titer for individual samples the seroprevalence was 50.1% and 33.4%, respectively while 59.9% were seropositive for pooled samples at HI . The correlation coefficients between pooled and individual samples at each submission were 0.592(p<0.001) for HI and 0.561(p<0.001) for , with common correlation coefficient of 0.576. This study indicated that pooled testing for the detection of IBV infection may be an alternative strategy when only the pooled results are of interest and the prevalence has not known exactly.
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KEYWORD
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sample size, pooled serum, chicken infectious bronchitis, seroprevalence
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